Reporter

Part:BBa_K567008:Design

Designed by: Yunfeng Ruan and Chunying Li   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)


PT7-Luc-2AGG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 911


Design Notes

PCR is used to insert the 2 AGG codons into the gene after ATG.


Source

T7 promoter is derived from pET28A. Wild type Luciferase from BBa_I712019.

References